OPTIMAL WNT AGONIST FOR HIGH LGR5+ INTESTINAL ORGANOID GROWTH AND FUNCTIONAL DIFFERENTIATED ORGANOID INDUCTION 

Background:

Wnt has been widely used as an essential factor in human intestinal organoids. Although recombinant Wnt3a is commonly utilized as Wnt source in organoid culture, a single Wnt3a protein is water insoluble and is not stable in culture medium. Recently, recombinant Afamin/Wnt3a and Wnt Surrogate-Fe Fusion Protein were developed. These Wnt agonists are stable as a medium supplement and support long-term organoid proliferation.