FAQs: QuickSwitch™ for MHC-peptide exchange

How many peptides can be exchanged and how many samples can be performed using 1 kit?

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What is the difference between QuickSwitch™ kit vs. QuickSwitch™ Quant kit?

What is the best fluorochrome?

What is the peptide concentration and purification needed for each antigen?

What is the exiting peptide that come with the QuickSwitch™ tetramer ?

How should I design the higher affinity peptide for peptide exchange using QuickSwitch™?

What is the best size of peptide sequence to use? Is there any specific requirement or limitation for peptide purity?

What peptide exchange percentage is acceptable for use in subsequent tetramer staining assays? 50%, 70% or higher?

What is your suggestion for the working concentration of lower affinity peptides using peptide exchange without tetramer aggregation?

What is the shelf life of resulting custom tetramer?

Can I use biotinylated antibodies with tetramers?

Do I need to use KT15 clone CD8 ab when I use the H-2Kb Allele QuickSwitch™ kit?

Do we need to desalt the tetramer after peptide exchange?

Can I use QuickSwitch™ non-exchange tetramer as negative control?

Do all QuickSwitch™ kits contain the same capture beads?

I'm using the same peptide as I used with a previous exchange, why is my exchange rate not as high?

Does the Human Class I QuickSwitch™ tetramer contain the α3 mutation?

I don't have a plate magnet. Can I still use your QuickSwitch™ kits?

Are there procedure differences between the kits?

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